Fig 1: Receiver-operating characteristic (ROC) analysis for S100A8 (a), S100A9 (b), TfR1 (c), and SAP (d) to discriminate PQ poisoning patients from healthy controls, respectively.
Fig 2: Serum expression of S100A8 (a), S100A9 (b), TfR1 (c), and SAP (d) detected by ELISA in the different clinical groups, respectively. Increased S100A8 and S100A9 serum levels were observed for all PQ poisoning patients (n = 21) compared to healthy controls (n = 21). Conversely, PQ poisoning groups showed significantly lower serum levels of TfR1 and SAP than the healthy group.
Fig 3: IHC analysis of S100A8 and S100A9 expression in lung tissue from PQ-treated rat and healthy controls. (a) and (b) Absence of staining for S100A8 and S100A9 in the normal lung tissue, respectively. (c) and (d) Marked S100A8 and S100A9 staining in lung tissue from rat treated with PQ. (Original magnification, 40×. Scale bars = 50 µm). Results of S100A8 (e) and S100A9 (f) immunohistochemistry in the cytoplasm and cell membrane in the lung tissues were quantitated by average optical of positive staining per 200 field.
Fig 4: MANF deficiency in myeloid cells activates S100A8/A9-TLR4 signal pathway in mice with liver fibrosis. (A) MANF deficiency in myeloid cells upregulated hepatic S100A8, S100A9, TLR4 and p-p65 levels detected by using immunohistochemistry assay. (B) The quantitative data in panel A. (C) S100A8, S100A9, TLR4, p-p65 and p65 were detected by Western blot. (D) The quantitative data in panel C. (E) S100a8, S100a9, and Tlr4 mRNA levels were detected by qPCR. (F) MANF deficiency in myeloid cells increased serum S100A8 and S100A9 levels. Serum S100A8 and S100A9 levels were detected by ELISA. Data are expressed as mean ± SEM, n = 10; ∗∗P < 0.01, ∗∗∗P < 0.001. WT, wild type; HKO, MANF knockout in hepatocytes; MKO, MANF knockout in myeloid cells; CCl4, carbon tetrachloride.
Fig 5: MANF interacts with S100A8 to impede S100A8/A9-TLR4 signaling. (A) Interaction of MANF and S100A8 was detected by Co-IP. (B) Colocalization of MANF (red) and S100A8 (green) was detected by immunofluorescent staining. DAPI (blue) was used to stain nuclei. (C, D) MANF overexpression inhibited intracellular (C) and extracellular (D) interaction of S100A9 and S100A8 detected by Co-IP with anti-S100A8. (E, F) MANF deficiency in macrophages increased colocalization of S100A9 and TLR4 in peritoneal macrophages (E) and hepatic macrophages (F) by detecting TLR4 (green) and S100A9 (red), n = 5. DAPI (blue) was used to stain nuclei. WT, wild type; MKO, MANF knockout in myeloid cells; LPS, lipopolysaccharide.
Supplier Page from Abcam for Mouse S100A9 ELISA Kit